"PFDN6" Related Products

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Recombinant Human PFDN6 293 Cell Lysate

Cat.No.: PFDN6-3277HCL
Description: Antigen standard for prefoldin subunit 6 (PFDN6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PFDN6 prefoldin subunit 6 [ Homo sapiens ]
Official Symbol: PFDN6
Synonyms: PFDN6; prefoldin subunit 6; HKE2, HLA class II region expressed gene KE2 , prefoldin 6; H2 KE2; KE 2; PFD6; KE2 protein; prefoldin 6; protein Ke2; HLA class II region expressed gene KE2; HKE2; KE-2; H2-KE2; MGC70744;
Gene ID: 10471
mRNA Refseq: NM_001185181
Protein Refseq: NP_001172110
MIM: 605660
UniProt ID: O15212
Chromosome Location: 6p21.3
Pathway: Chaperonin-mediated protein folding, organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Prefoldin mediated transfer of substrateto CCT/TriC, organism-specific biosystem; Protein folding, organism-specific biosystem;
Function: chaperone binding; unfolded protein binding;

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