Recombinant Human PDE6D 293 Cell Lysate

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Recombinant Human PDE6D 293 Cell Lysate

PDE6D Related Products

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Cat.No. : PDE6D-3345HCL
Description : Antigen standard for phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PDE6D phosphodiesterase 6D, cGMP-specific, rod, delta [ Homo sapiens ]
Official Symbol : PDE6D
Synonyms : PDE6D; phosphodiesterase 6D, cGMP-specific, rod, delta; retinal rod rhodopsin-sensitive cGMP 3,5-cyclic phosphodiesterase subunit delta; protein p17; GMP-PDE delta; PDED;
Gene ID : 5147
mRNA Refseq : NM_002601
Protein Refseq : NP_002592
MIM : 602676
UniProt ID : O43924
Chromosome Location : 2q35-q36
Pathway : Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem;
Function : 3,5-cyclic-nucleotide phosphodiesterase activity; GTPase inhibitor activity; protein binding;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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