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Recombinant Human PCYT2 293 Cell Lysate

Cat.No. : PCYT2-3365HCL
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Description : Antigen standard for phosphate cytidylyltransferase 2, ethanolamine (PCYT2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PCYT2 phosphate cytidylyltransferase 2, ethanolamine [ Homo sapiens ]
Official Symbol : PCYT2
Synonyms : PCYT2; phosphate cytidylyltransferase 2, ethanolamine; ethanolamine-phosphate cytidylyltransferase; ET; phosphorylethanolamine transferase; CTP:phosphoethanolamine cytidylyltransferase;
Gene ID : 5833
mRNA Refseq : NM_001184917
Protein Refseq : NP_001171846
MIM : 602679
UniProt ID : Q99447
Chromosome Location : 17q25.3
Pathway : Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Phosphatidylethanolamine (PE) biosynthesis, ethanolamine =>PE, organism-specific biosystem; Phosphatidylethanolamine (PE) biosynthesis, ethanolamine => PE, conserved biosystem;
Function : ethanolamine-phosphate cytidylyltransferase activity; nucleotidyltransferase activity; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the significance of PCYT2 research for agricultural production? 05/14/2023

The study of PCYT2 can help us better understand the regulatory mechanism of plant growth and development, and provide new ideas and methods for agricultural production, such as improving crop yield and stress resistance by regulating the expression level of PCYT2.

What aspects should future research on PCYT2 focus on? 04/05/2023

Future research on PCYT2 may focus on the following aspects: the relationship between its structure and function, the interaction mechanism with other molecules, the mechanism of action in plant response to environmental stimuli, and the application in agricultural production.

What is the effect of genetic variation of PCYT2 in plants? 09/30/2021

Genetic variation of PCYT2 in plants may affect its function, thus affecting plant growth and development and environmental adaptability.

How does PCYT2 respond to environmental stimuli? 08/25/2021

PCYT2 responds to environmental stimuli such as drought and high temperatures by regulating protein synthesis in response to these challenges.

How is the expression level of PCYT2 regulated? 12/16/2020

The expression level of PCYT2 can be regulated in a variety of ways, including transcription, post-transcriptional modification, and degradation.

How does PCYT2 interact with other molecules? 10/21/2020

PCYT2 can interact with other molecules, such as other trnas, ribosome subunits, and translation factors, to regulate protein synthesis.

Customer Reviews (3)

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Reviews
11/17/2021

    Kinetic parameters such as enzymatic reaction rate and binding affinity were tested in the early stage, and it was found that this protein performed well.

    09/03/2020

      With PCYT2, the reproducibility of the experiment was significantly improved.

      01/09/2020

        Good stability under different temperature and humidity conditions.

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