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Recombinant Human PCK1 293 Cell Lysate

Cat.No. : PCK1-3379HCL
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  • Gene Information
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Description : Antigen standard for phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PCK1 phosphoenolpyruvate carboxykinase 1 (soluble) [ Homo sapiens ]
Official Symbol : PCK1
Synonyms : PCK1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK C; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase; phosphoenolpyruvate carboxykinase, cytosolic; PEPCK1; PEPCKC; PEPCK-C; MGC22652;
Gene ID : 5105
mRNA Refseq : NM_002591
Protein Refseq : NP_002582
MIM : 614168
UniProt ID : P35558
Chromosome Location : 20q13.31
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem;
Function : GDP binding; GTP binding; carboxylic acid binding; lyase activity; magnesium ion binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; purine nucleotide binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What new mechanisms or functions have been discovered in recent studies on PCK1? 12/07/2022

Recent studies have found that PCK1 is also involved in the synthesis and oxidation of fatty acids. These processes have an important impact on energy metabolism and cell growth, and also provide new ideas for the treatment of some PCK1-related diseases.

Why are studies of PCK1 limited? 12/03/2021

There are still some limitations in the current research on PCK1, such as the lack of in-depth research on its specific mechanism of action and function, and the incomplete understanding of its relationship with diseases. In addition, since PCK1 is a key enzyme in the gluconeogenesis pathway, its regulation may also have some side effects.

What is the role of PCK1 in energy metabolism? 09/15/2021

PCK1 plays a key role in energy metabolism. By participating in the gluconeogenesis pathway, it maintains a stable supply of glucose in the body, thereby ensuring the body's energy needs in stressful states such as hunger and exercise.

How is the activity of PCK1 regulated? 04/11/2021

The activity of PCK1 is regulated by a variety of factors, including hormones (such as insulin, glucagon, etc.), nutrients (such as glucose, fatty acids, etc.), and intracellular signal transduction pathways (such as AMPK, mTOR, etc.). These factors regulate the activity of PCK1 by influencing its phosphorylation, acetylation and other modifications.

How can I detect the expression level of PCK1? 10/18/2020

The expression level of PCK1 can be detected by enzyme-linked immunosorbent assay, immunohistochemistry, real-time PCR and other methods. These methods can detect protein or mRNA levels of PCK1 in tissues or cells to assess changes in its expression in disease.

What are the treatment strategies for PCK1? 08/30/2020

Therapeutic strategies for PCK1 include inhibiting its expression, modulating its activity, or using gene therapy. For example, some glucose-lowering drugs (such as metformin) reduce blood sugar levels by inhibiting the activity of PCK1 and reducing the output of glucose in the liver. In addition, it is also possible to treat related diseases by regulating the expression level of PCK1.

Customer Reviews (3)

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Reviews
05/18/2023

    It has good solubility and can be easily dissolved.

    05/12/2021

      I performed well when doing experiments such as SDS-PAGE, and the results were great.

      04/20/2021

        The synthesis efficiency is high, the yield is high, and the stability is good.

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