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Recombinant Human PCBP4 293 Cell Lysate

Cat.No. : PCBP4-3401HCL
  • Specification
  • Gene Information
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Description : Antigen standard for poly(rC) binding protein 4 (PCBP4), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PCBP4 poly(rC) binding protein 4 [ Homo sapiens ]
Official Symbol : PCBP4
Synonyms : PCBP4; poly(rC) binding protein 4; poly(rC)-binding protein 4; alphaCP 4 protein; LIP4; LYST interacting protein; MCG10; RNA binding protein MCG10; alpha-CP4; LYST-interacting protein; CBP;
Gene ID : 57060
mRNA Refseq : NM_001174100
Protein Refseq : NP_001167571
MIM : 608503
UniProt ID : P57723
Chromosome Location : 3p21
Pathway : Direct p53 effectors, organism-specific biosystem;
Function : DNA binding; RNA binding; RNA binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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