"PAMR1" Related Products

Recombinant Human PAMR1 293 Cell Lysate

Cat.No.: PAMR1-3447HCL
Description: Antigen standard for peptidase domain containing associated with muscle regeneration 1 (PAMR1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PAMR1 peptidase domain containing associated with muscle regeneration 1 [ Homo sapiens ]
Official Symbol: PAMR1
Synonyms: PAMR1; peptidase domain containing associated with muscle regeneration 1; inactive serine protease PAMR1; DKFZP586H2123; RAMP; regeneration associated muscle protease; regeneration-associated muscle protease homolog; peptidase domain-containing protein associated with muscle regeneration 1; FP938;
Gene ID: 25891
mRNA Refseq: NM_001001991
Protein Refseq: NP_001001991
UniProt ID: Q6UXH9
Chromosome Location: 11p13
Function: catalytic activity; serine-type endopeptidase activity;
Products Types ◆ Recombinant Protein

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