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Recombinant Human PAFAH1B2 293 Cell Lysate

Cat.No.: PAFAH1B2-3468HCL
Description: Antigen standard for platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa) (PAFAH1B2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PAFAH1B2 platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa) [ Homo sapiens ]
Official Symbol: PAFAH1B2
Synonyms: PAFAH1B2; platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa); platelet activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa , platelet activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa); platelet-activating factor acetylhydrolase IB subunit beta; PAF AH1b alpha 2 subunit; PAFAH subunit beta; PAF-AH subunit beta; PAF-AH 30 kDa subunit; PAF-AH1b alpha 2 subunit; PAF acetylhydrolase 30 kDa subunit; intracellular platelet-activating factor acetylhydrolase alpha 2 subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa);
Gene ID: 5049
mRNA Refseq: NM_001184746
Protein Refseq: NP_001171675
MIM: 602508
UniProt ID: P68402
Chromosome Location: 11q23
Pathway: Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Lissencephaly gene (LIS1) in neuronal migration and development, organism-specific biosystem; Metabolic pathways, organism-specific biosystem;
Function: 1-alkyl-2-acetylglycerophosphocholine esterase activity; hydrolase activity;

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