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Recombinant Human PA2G4 293 Cell Lysate

Cat.No. : PA2G4-3478HCL
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  • Gene Information
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Description : Antigen standard for proliferation-associated 2G4, 38kDa (PA2G4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PA2G4 proliferation-associated 2G4, 38kDa [ Homo sapiens ]
Official Symbol : PA2G4
Synonyms : PA2G4; proliferation-associated 2G4, 38kDa; proliferation associated 2G4, 38kD; proliferation-associated protein 2G4; erbB3-binding protein 1; ErbB-3 binding protein 1; ErbB3-binding protein Ebp1; cell cycle protein p38-2G4 homolog; EBP1; HG4-1; p38-2G4;
Gene ID : 5036
mRNA Refseq : NM_006191
Protein Refseq : NP_006182
MIM : 602145
UniProt ID : Q9UQ80
Chromosome Location : 12q13.2
Pathway : Androgen Receptor Signaling Pathway, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem;
Function : DNA binding; RNA binding; protein binding; sequence-specific DNA binding transcription factor activity; ubiquitin protein ligase binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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