"OTP" Related Products

Recombinant Human OTP 293 Cell Lysate

Cat.No.: OTP-3517HCL
Description: Antigen standard for orthopedia homeobox (OTP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: OTP orthopedia homeobox [ Homo sapiens ]
Official Symbol: OTP
Synonyms: OTP; orthopedia homeobox; orthopedia homolog (Drosophila); homeobox protein orthopedia; orthopedia homolog; MGC3161;
Gene ID: 23440
mRNA Refseq: NM_032109
Protein Refseq: NP_115485
MIM: 604529
UniProt ID: Q5XKR4
Chromosome Location: 5q14.1
Function: transcription regulatory region sequence-specific DNA binding;
Products Types ◆ Recombinant Protein

Related Gene
OTP

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