"ORC1L" Related Products

Recombinant Human ORC1L 293 Cell Lysate

Cat.No.: ORC1L-3553HCL
Description: Antigen standard for origin recognition complex, subunit 1-like (yeast) (ORC1L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ORC1 origin recognition complex, subunit 1 [ Homo sapiens(human) ]
Official Symbol: ORC1L
Synonyms: ORC1; ORC1L; PARC1; HSORC1; origin recognition complex, subunit 1; origin recognition complex subunit 1; replication control protein 1; origin recognition complex, subunit 1 homolog
Gene ID: 4998
mRNA Refseq: NM_001190818
Protein Refseq: NP_001177747
MIM: 601902
UniProt ID: Q13415
Chromosome Location: 1p32
Pathway: Activation of ATR in response to replication stress; Activation of the pre-replicative complex; Assembly of the pre-replicative complex
Function: ATP binding; DNA binding; nucleoside-triphosphatase activity

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