Recombinant Human OR2F1 293 Cell Lysate

• Cat.No.: OR2F1-3564HCL

Specification

• Description: Antigen standard for olfactory receptor, family 2, subfamily F, member 1 (OR2F1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: OR2F1 olfactory receptor, family 2, subfamily F, member 1 [ Homo sapiens ]
• Official Symbol: OR2F1
• Synonyms: OR2F1; olfactory receptor, family 2, subfamily F, member 1; OR2F3, OR2F3P, OR2F4, OR2F5; olfactory receptor 2F1; OLF3; OR7 139; OR7 140; OR14 60; olfactory receptor 2F3; olfactory receptor 2F4; olfactory receptor 2F5; olfactory receptor OR7-7; olfactory receptor-like protein OLF3; olfactory receptor, family 2, subfamily F, member 3; olfactory receptor, family 2, subfamily F, member 4; olfactory receptor, family 2, subfamily F, member 5; OR2F3; OR2F4; OR2F5; OR2F3P; OR14-60; OR7-139; OR7-140
• Gene ID: 26211
• mRNA Refseq: NM_012369
• Protein Refseq: NP_036501
• MIM: 608497
• UniProt ID: Q13607
• Chromosome Location: 7q35
• Pathway: GPCR downstream signaling, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; GPCRs, Other, organism-specific biosystem; Olfactory Signaling Pathway, organism-specific biosystem; Olfactory transduction, organism-specific biosystem; Olfactory transduction, conserved biosystem; Signal Transduction, organism-specific biosystem
• Function: G-protein coupled receptor activity; olfactory receptor activity; receptor activity; signal transducer activity