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Recombinant Human OIP5 293 Cell Lysate

Cat.No. : OIP5-3588HCL
  • Specification
  • Gene Information
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Description : Antigen standard for Opa interacting protein 5 (OIP5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : OIP5 Opa interacting protein 5 [ Homo sapiens ]
Official Symbol : OIP5
Synonyms : OIP5; Opa interacting protein 5; protein Mis18-beta; cancer/testis antigen 86; CT86; hMIS18beta; MIS18 kinetochore protein homolog B (S. pombe); MIS18B; OIP-5; LAP2alpha interactor-25; opa-interacting protein 5; MIS18 kinetochore protein homolog B; LINT-25; MIS18beta; 5730547N13Rik;
Gene ID : 11339
mRNA Refseq : NM_007280
Protein Refseq : NP_009211
MIM : 606020
UniProt ID : O43482
Chromosome Location : 15q14
Pathway : Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Deposition of New CENPA-containing Nucleosomes at the Centromere, organism-specific biosystem; Nucleosome assembly, organism-specific biosystem;
Function : protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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