"ODF4" Related Products

Recombinant Human ODF4 293 Cell Lysate

Cat.No.: ODF4-3594HCL
Description: Antigen standard for outer dense fiber of sperm tails 4 (ODF4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ODF4 outer dense fiber of sperm tails 4 [ Homo sapiens ]
Official Symbol: ODF4
Synonyms: ODF4; outer dense fiber of sperm tails 4; outer dense fiber protein 4; cancer/testis antigen 136; CT136; OPPO1; hOPPO1; outer dense fiber 4; cancer/testis antigen 134; testis-specific protein oppo 1; outer dense fiber of sperm tails protein 4; CT134; MGC138215; MGC138219;
Gene ID: 146852
mRNA Refseq: NM_153007
Protein Refseq: NP_694552
MIM: 610097
UniProt ID: Q2M2E3
Chromosome Location: 17p13

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