Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human NUAK1 293 Cell Lysate

Cat.No. : NUAK1-3665HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for NUAK family, SNF1-like kinase, 1 (NUAK1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : NUAK1 NUAK family, SNF1-like kinase, 1 [ Homo sapiens ]
Official Symbol : NUAK1
Synonyms : NUAK1; NUAK family, SNF1-like kinase, 1; NUAK family SNF1-like kinase 1; AMP activated protein kinase family member 5; ARK5; KIAA0537; NuaK1; omphalocele kinase 1; AMPK-related protein kinase 5; AMP-activated protein kinase family member 5;
Gene ID : 9891
mRNA Refseq : NM_014840
Protein Refseq : NP_055655
MIM : 608130
UniProt ID : O60285
Chromosome Location : 12q23.3
Function : ATP binding; metal ion binding; nucleotide binding; p53 binding; protein binding; protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Are there any genetic mutations associated with NUAK1 protein that have clinical implications? 08/27/2019

Mutations in the NUAK1 gene have been identified in certain cancers and may have implications for prognosis and treatment response.

Are there any drugs targeting NUAK1 protein in cancer treatment? 06/15/2018

Yes, several preclinical studies have identified potential drug candidates that target NUAK1 protein for cancer therapy.

How does NUAK1 protein affect stress responses in cells? 01/28/2018

NUAK1 protein has been shown to regulate stress responses in cells, including responses to oxidative stress and DNA damage.

Are there any side effects associated with targeting NUAK1 protein in clinical applications? 01/28/2017

Potential side effects may include off-target effects on other cellular processes and toxicity to normal cells.

What are the potential therapeutic implications of targeting NUAK1 protein in stress-related conditions? 09/17/2016

Targeting NUAK1 protein may have implications for conditions related to stress responses, such as neurodegenerative diseases and aging-related disorders.

Customer Reviews (3)

Write a review
Reviews
12/15/2018

    The NUAK1 protein is an exceptional product that meets the highest standards of quality, making it an ideal choice to fulfill my experimental requirements.

    05/06/2017

      By incorporating the NUAK1 protein into electron microscopy studies, researchers gain insights into the intricate three-dimensional architecture of proteins, facilitating a deeper comprehension of their functions and aiding in the development of novel therapeutics.

      03/14/2016

        Its reliability and effectiveness in generating accurate data make it an indispensable resource, driving scientific exploration and advancing our understanding of protein structure and function

        Ask a Question for All NUAK1 Products

        Required fields are marked with *

        My Review for All NUAK1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends