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Recombinant Human NRBP1 293 Cell Lysate

Cat.No.: NRBP1-3701HCL
Description: Antigen standard for nuclear receptor binding protein 1 (NRBP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NRBP1 nuclear receptor binding protein 1 [ Homo sapiens ]
Official Symbol: NRBP1
Synonyms: NRBP1; nuclear receptor binding protein 1; NRBP, nuclear receptor binding protein; nuclear receptor-binding protein; BCON3; MADM; MUDPNP; multiple domain putative nuclear protein; myeloid leukemia factor 1 adaptor molecule; NRBP; FLJ27109; FLJ35541;
Gene ID: 29959
mRNA Refseq: NM_013392
Protein Refseq: NP_037524
MIM: 606010
UniProt ID: Q9UHY1
Chromosome Location: 2p23
Pathway: Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem;
Function: ATP binding; protein binding; protein homodimerization activity; protein kinase activity; transferase activity, transferring phosphorus-containing groups;

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