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Recombinant Human NR2C1 293 Cell Lysate

Cat.No.: NR2C1-3714HCL
Description: Antigen standard for nuclear receptor subfamily 2, group C, member 1 (NR2C1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NR2C1 nuclear receptor subfamily 2, group C, member 1 [ Homo sapiens ]
Official Symbol: NR2C1
Synonyms: NR2C1; nuclear receptor subfamily 2, group C, member 1; TR2; nuclear receptor subfamily 2 group C member 1; TR2 11; orphan nuclear receptor TR2; TR2 nuclear hormone receptor; nuclear receptor subfamily 2, group C isoform;
Gene ID: 7181
mRNA Refseq: NM_001032287
Protein Refseq: NP_001027458
MIM: 601529
UniProt ID: P13056
Chromosome Location: 12q22
Pathway: Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem;
Function: DNA binding; histone deacetylase binding; metal ion binding; protein binding; protein homodimerization activity; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; zinc ion binding;

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