"NFX1" Related Products

Recombinant Human NFX1 293 Cell Lysate

Cat.No.: NFX1-3842HCL
Description: Antigen standard for nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NFX1 nuclear transcription factor, X-box binding 1 [ Homo sapiens ]
Official Symbol: NFX1
Synonyms: NFX1; nuclear transcription factor, X-box binding 1; transcriptional repressor NF-X1; MGC20369; NFX2; nuclear transcription factor, X box-binding protein 1; DKFZp779G2416;
Gene ID: 4799
mRNA Refseq: NM_002504
Protein Refseq: NP_002495
MIM: 603255
UniProt ID: Q12986
Chromosome Location: 9p12
Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem; Transport of Mature Transcript to Cytoplasm, organism-specific biosystem; Transport of Mature mRNA derived from an Intron-Containing Transcript, organism-specific biosystem;
Function: DNA binding; ligase activity; metal ion binding; sequence-specific DNA binding transcription factor activity; zinc ion binding;
Products Types ◆ Recombinant Protein

Related Gene

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.