"NFS1" Related Products

Recombinant Human NFS1 293 Cell Lysate

Cat.No.: NFS1-3844HCL
Description: Antigen standard for NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NFS1 NFS1 nitrogen fixation 1 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: NFS1
Synonyms: NFS1; NFS1 nitrogen fixation 1 homolog (S. cerevisiae); nitrogen fixation 1 (S. cerevisiae, homolog); cysteine desulfurase, mitochondrial; IscS; NifS; nitrogen-fixing bacteria S-like protein; NIFS; HUSSY-08;
Gene ID: 9054
mRNA Refseq: NM_001198989
Protein Refseq: NP_001185918
MIM: 603485
UniProt ID: Q9Y697
Chromosome Location: 20q11.22
Pathway: Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; Molybdenum cofactor biosynthesis, organism-specific biosystem; Sulfur relay system, organism-specific biosystem; Sulfur relay system, conserved biosystem; Thiamine metabolism, organism-specific biosystem;
Function: catalytic activity; cysteine desulfurase activity; cysteine desulfurase activity; protein binding; protein homodimerization activity; pyridoxal phosphate binding; transferase activity;

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