Recombinant Human NET1 293 Cell Lysate

• Cat.No.: NET1-3873HCL

Specification

• Description: Antigen standard for neuroepithelial cell transforming 1 (NET1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NET1 neuroepithelial cell transforming 1 [ Homo sapiens ]
• Official Symbol: NET1
• Synonyms: NET1; neuroepithelial cell transforming 1; neuroepithelial cell-transforming gene 1 protein; ARHGEF8; NET1A; proto-oncogene p65 Net1; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator; neuroepithelioma transforming gene 1; neuroepithelial cell transforming gene 1; rho guanine nucleotide exchange factor 8; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene)
• Gene ID: 10276
• mRNA Refseq: NM_001047160
• Protein Refseq: NP_001040625
• MIM: 606450
• UniProt ID: Q7Z628
• Chromosome Location: 10p15
• Pathway: Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; G alpha (12/13) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; NRAGE signals death through JNK, organism-specific biosystem; Regulation of RhoA activity, organism-specific biosystem; Rho GTPase cycle, organism-specific biosystem; Signal Transduction, organism-specific biosystem
• Function: Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity