"NEIL1" Related Products

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Recombinant Human NEIL1 293 Cell Lysate

Cat.No.: NEIL1-3882HCL
Description: Antigen standard for nei endonuclease VIII-like 1 (E. coli) (NEIL1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NEIL1 nei endonuclease VIII-like 1 (E. coli) [ Homo sapiens ]
Official Symbol: NEIL1
Synonyms: NEIL1; nei endonuclease VIII-like 1 (E. coli); endonuclease 8-like 1; FLJ22402; FPG1; hFPG1; NEI1; NEH1; nei homolog 1; endonuclease VIII; nei-like protein 1; endonuclease VIII-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1;
Gene ID: 79661
mRNA Refseq: NM_001256552
Protein Refseq: NP_001243481
MIM: 608844
UniProt ID: Q96FI4
Chromosome Location: 15q33.33
Pathway: Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem;
Function: DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; damaged DNA binding; hydrolase activity, acting on glycosyl bonds; lyase activity; protein C-terminus binding; zinc ion binding;

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