Recombinant Human NDUFS1 293 Cell Lysate

• Cat.No.: NDUFS1-3898HCL

Specification

• Description: Antigen standard for NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NDUFS1 NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) [ Homo sapiens ]
• Official Symbol: NDUFS1
• Synonyms: NDUFS1; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); NADH dehydrogenase (ubiquinone) Fe S protein 1 (75kD) (NADH coenzyme Q reductase); NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; CI 75k; complex I 75kDa subunit; NADH ubiquinone oxidoreductase 75 kDa subunit; mitochondrial; complex I, mitochondrial respiratory chain, 75-kD subunit; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit; CI-75k; CI-75Kd; PRO1304; MGC26839
• Gene ID: 4719
• mRNA Refseq: NM_001199981
• Protein Refseq: NP_001186910
• MIM: 157655
• UniProt ID: P28331
• Chromosome Location: 2q33-q34
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: 2 iron, 2 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; contributes_to NADH dehydrogenase (ubiquinone) activity; NADH dehydrogenase (ubiquinone) activity; NADH dehydrogenase activity; electron carrier activity; metal ion binding; protein binding