"MTRF1" Related Products


Recombinant Human MTRF1 293 Cell Lysate

Cat.No.: MTRF1-4067HCL
Description: Antigen standard for mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MTRF1 mitochondrial translational release factor 1 [ Homo sapiens ]
Official Symbol: MTRF1
Synonyms: MTRF1; mitochondrial translational release factor 1; peptide chain release factor 1, mitochondrial; MGC47721; mitochontrial peptide chain release factor 1; MTTRF1; RF1; MRF-1; mtRF-1; MRF1;
Gene ID: 9617
mRNA Refseq: NM_004294
Protein Refseq: NP_004285
MIM: 604601
UniProt ID: O75570
Chromosome Location: 13q14.1-q14.3
Function: translation release factor activity; translation release factor activity, codon specific;

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