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Recombinant Human MSH6 293 Cell Lysate

Cat.No. : MSH6-4117HCL
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  • Gene Information
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Description : Antigen standard for mutS homolog 6 (E. coli) (MSH6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : MSH6 mutS homolog 6 (E. coli) [ Homo sapiens ]
Official Symbol : MSH6
Synonyms : MSH6; mutS homolog 6 (E. coli); GTBP, mutS (E. coli) homolog 6; DNA mismatch repair protein Msh6; p160; GTMBP; hMSH6; sperm-associated protein; mutS-alpha 160 kDa subunit; G/T mismatch-binding protein; GTBP; HSAP; HNPCC5;
Gene ID : 2956
mRNA Refseq : NM_000179
Protein Refseq : NP_000170
MIM : 600678
UniProt ID : P52701
Chromosome Location : 2p16
Pathway : BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; Colorectal cancer, organism-specific biosystem; Colorectal cancer, conserved biosystem; Mismatch repair, organism-specific biosystem; Mismatch repair, conserved biosystem; Pathways in cancer, organism-specific biosystem;
Function : contributes_to ADP binding; contributes_to ATP binding; contributes_to ATPase activity; DNA-dependent ATPase activity; contributes_to MutLalpha complex binding; chromatin binding; damaged DNA binding; contributes_to double-stranded DNA binding; contributes_to four-way junction DNA binding; contributes_to guanine/thymine mispair binding; contributes_to magnesium ion binding; contributes_to mismatched DNA binding; nucleotide binding; contributes_to oxidized purine DNA binding; protein binding; contributes_to protein binding; NOT protein homodimerization activity; contributes_to single guanine insertion binding; contributes_to single thymine insertion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What are the mechanisms by which MSH6 modulates cell cycle checkpoints and apoptosis? 10/15/2021

MSH6 modulates cell cycle and apoptosis, ensuring cells with DNA damage do not proliferate.

What is the impact of MSH6 mutations on the risk of other types of cancers? 05/02/2021

Mutations in MSH6 increase the risk of various cancers, due to impaired DNA repair mechanisms.

How does MSH6 deficiency contribute to the development of hereditary non-polyposis colorectal cancer (HNPCC)? 02/10/2021

MSH6 deficiency can lead to HNPCC by allowing accumulation of DNA replication errors.

How does MSH6 (MutS Homolog 6) function in the DNA mismatch repair system? 07/10/2020

MSH6 is crucial in DNA mismatch repair, helping correct replication errors to maintain DNA integrity.

What role does MSH6 play in maintaining genomic stability and preventing mutations? 06/19/2020

MSH6 plays a vital role in preventing mutations and maintaining genomic stability.

How do alterations in MSH6 expression or function affect the efficacy of chemotherapy in cancer treatment? 05/06/2018

Alterations in MSH6 can affect the response to chemotherapy, potentially impacting treatment outcomes.

How does MSH6 influence the cellular response to DNA damage and repair processes? 02/09/2018

MSH6 is involved in the cellular response to DNA damage, facilitating repair to prevent mutations.

Customer Reviews (3)

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Reviews
02/01/2020

    Reliable mass spectrometry analysis, pivotal for advancing our proteomics research.

    08/29/2019

      High-quality protein crystallography services, consistently delivers exceptional results.

      02/19/2019

        Swift and reliable protein sequencing, a key asset for our work.

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