"MRFAP1" Related Products

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Recombinant Human MRFAP1 293 Cell Lysate

Cat.No.: MRFAP1-4209HCL
Description: Antigen standard for Mof4 family associated protein 1 (MRFAP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MRFAP1 Morf4 family associated protein 1 [ Homo sapiens ]
Official Symbol: MRFAP1
Synonyms: MRFAP1; Morf4 family associated protein 1; MORF4 family-associated protein 1; PAM14; PGR1; T-cell activation protein; Mof4 family associated protein 1; protein associated with MRG, 14 kDa; protein associated with MRG of 14 kDa;
Gene ID: 93621
mRNA Refseq: NM_033296
Protein Refseq: NP_150638
UniProt ID: Q9Y605
Chromosome Location: 4p16.1
Function: protein binding;

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