"MRAS" Related Products

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Recombinant Human MRAS 293 Cell Lysate

Cat.No.: MRAS-4213HCL
Description: Antigen standard for muscle RAS oncogene homolog (MRAS), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MRAS muscle RAS oncogene homolog [ Homo sapiens ]
Official Symbol: MRAS
Synonyms: MRAS; muscle RAS oncogene homolog; ras-related protein M-Ras; M RAs; R RAS3; RRAS3; muscle and microspikes RAS; ras-related protein R-Ras3; M-RAs; R-RAS3; FLJ42964;
Gene ID: 22808
mRNA Refseq: NM_001085049
Protein Refseq: NP_001078518
MIM: 608435
UniProt ID: O14807
Chromosome Location: 3q22.3
Pathway: HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL-3 Signaling Pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Regulation of Actin Cytoskeleton, organism-specific biosystem;
Function: GTP binding; GTP-dependent protein binding; GTPase activity; nucleotide binding;

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