Recombinant Human MOBKL1A 293 Cell Lysate


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Recombinant Human MOBKL1A 293 Cell Lysate

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Cat.No. : MOBKL1A-4266HCL
Description : Antigen standard for MOB1, Mps One Binder kinase activator-like 1A (yeast) (MOBKL1A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : MOB1B MOB kinase activator 1B [ Homo sapiens (human) ]
Official Symbol : MOBKL1A
Synonyms : MOB1B; MATS2; MOB4A; MOBKL1A; MOB kinase activator 1B; mob1A; mob1 homolog 1A; MOB1 Mps One Binder homolog B; mps one binder kinase activator-like 1A; MOB1, Mps One Binder kinase activator-like 1A
Gene ID : 92597
mRNA Refseq : NM_001244766
Protein Refseq : NP_001231695
MIM : 609282
UniProt ID : Q7L9L4
Chromosome Location : 4q13.3
Pathway : Hippo signaling pathway; Signal Transduction; Signaling by Hippo
Function : kinase activator activity; kinase binding; metal ion binding

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