"MIF4GD" Related Products

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Recombinant Human MIF4GD 293 Cell Lysate

Cat.No.: MIF4GD-4315HCL
Description: Antigen standard for MIF4G domain containing (MIF4GD) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MIF4GD MIF4G domain containing [ Homo sapiens ]
Official Symbol: MIF4GD
Synonyms: MIFD; AD023; SLIP1; MIF4G domain-containing protein; SLBP-interacting protein 1; SLBP (stem loop binding protein)-interacting protein 1
Gene ID: 57409
mRNA Refseq: NM_001242498
Protein Refseq: NP_001229427
MIM: 612072
UniProt ID: A9UHW6
Chromosome Location: 17q25.1
Function: RNA binding; protein C-terminus binding; protein binding

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