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Recombinant Human MEF2D 293 Cell Lysate

Cat.No.: MEF2D-4374HCL
Description: Antigen standard for myocyte enhancer factor 2D (MEF2D) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MEF2D myocyte enhancer factor 2D [ Homo sapiens ]
Official Symbol: MEF2D
Synonyms: MEF2D; myocyte enhancer factor 2D; myocyte-specific enhancer factor 2D; MEF2D/DAZAP1 fusion; MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancer factor 2D); myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusion protein; DKFZp686I1536;
Gene ID: 4209
mRNA Refseq: NM_005920
Protein Refseq: NP_005911
MIM: 600663
UniProt ID: Q14814
Chromosome Location: 1q12-q23
Pathway: Adipogenesis, organism-specific biosystem; CDO in myogenesis, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; Myogenesis, organism-specific biosystem; Role of Calcineurin-dependent NFAT signaling in lymphocytes, organism-specific biosystem;
Function: DNA binding; RNA polymerase II regulatory region sequence-specific DNA binding; activating transcription factor binding; histone deacetylase binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity;

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