Recombinant Human MB 293 Cell Lysate
Cat.No. : | MB-4446HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for myoglobin (MB), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | MB myoglobin [ Homo sapiens ] |
Official Symbol : | MB |
Synonyms : | MB; myoglobin; PVALB; MGC13548; |
Gene ID : | 4151 |
mRNA Refseq : | NM_005368 |
Protein Refseq : | NP_005359 |
MIM : | 160000 |
UniProt ID : | P02144 |
Chromosome Location : | 22q13.1 |
Function : | heme binding; metal ion binding; oxygen binding; oxygen transporter activity; |
Products Types
◆ Recombinant Protein | ||
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MB-0028H | Recombinant Human MB Protein | +Inquiry |
MB-2522H | Recombinant Human MB protein(21-100 aa), C-His-tagged | +Inquiry |
MB-25B | Active Native Bovine Myoglobin | +Inquiry |
MB-1768H | Recombinant Human MB Protein, His&GST-tagged | +Inquiry |
◆ Native Protein | ||
MB-4460H | Native Human Myoglobin | +Inquiry |
MB-236B | Native Bovine Myoglobin | +Inquiry |
Mb-160M | Native Mouse Mb | +Inquiry |
◆ Lysates | ||
MB-4445HCL | Recombinant Human MB 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMyoglobin's structure, with a single heme group, allows it to bind oxygen tightly, facilitating its role in oxygen storage and release in muscle tissues.
Myoglobin is released into the bloodstream when muscle cells are damaged. In the context of a myocardial infarction (heart attack), damaged heart muscle cells release myoglobin, making it a quick, albeit non-specific, marker for muscle injury.
Myoglobin's affinity for oxygen remains relatively constant across various environmental conditions, ensuring a steady oxygen supply for muscles even under stress.
While mutations in the MB gene are rare, they can lead to myoglobinuria, a condition where myoglobin is present in the urine, often after intense muscle activity.
Elevated myoglobin levels in blood tests can indicate muscle injury, including myocardial infarction. However, since myoglobin is also found in skeletal muscles, it is often used in conjunction with other markers to diagnose heart attacks.
While both myoglobin and hemoglobin bind oxygen, myoglobin has a higher affinity for oxygen and does not exhibit cooperative binding. This allows myoglobin to release oxygen under conditions where hemoglobin cannot, such as during intense muscle activity.
The MB gene encodes the protein myoglobin, which is responsible for storing and releasing oxygen in muscle cells, especially in conditions of high oxygen demand.
Customer Reviews (3)
Write a reviewEffective in pull-down assay.
Perfect for my experiments.
High-quality product, very satisfied.
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