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Recombinant Human MB 293 Cell Lysate

Cat.No. : MB-4446HCL
  • Specification
  • Gene Information
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Description : Antigen standard for myoglobin (MB), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : MB myoglobin [ Homo sapiens ]
Official Symbol : MB
Synonyms : MB; myoglobin; PVALB; MGC13548;
Gene ID : 4151
mRNA Refseq : NM_005368
Protein Refseq : NP_005359
MIM : 160000
UniProt ID : P02144
Chromosome Location : 22q13.1
Function : heme binding; metal ion binding; oxygen binding; oxygen transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does the structure of myoglobin facilitate its function in muscle tissues? 02/14/2022

Myoglobin's structure, with a single heme group, allows it to bind oxygen tightly, facilitating its role in oxygen storage and release in muscle tissues.

Why is myoglobin considered a marker for muscle injury, especially in the context of myocardial infarction? 11/19/2021

Myoglobin is released into the bloodstream when muscle cells are damaged. In the context of a myocardial infarction (heart attack), damaged heart muscle cells release myoglobin, making it a quick, albeit non-specific, marker for muscle injury.

How is myoglobin's affinity for oxygen influenced by environmental conditions? 09/14/2021

Myoglobin's affinity for oxygen remains relatively constant across various environmental conditions, ensuring a steady oxygen supply for muscles even under stress.

Are there any known diseases or conditions associated with mutations or dysregulation of MB? 07/05/2020

While mutations in the MB gene are rare, they can lead to myoglobinuria, a condition where myoglobin is present in the urine, often after intense muscle activity.

What are the clinical implications of elevated myoglobin levels in blood tests? 01/23/2019

Elevated myoglobin levels in blood tests can indicate muscle injury, including myocardial infarction. However, since myoglobin is also found in skeletal muscles, it is often used in conjunction with other markers to diagnose heart attacks.

How does myoglobin differ from hemoglobin in terms of oxygen binding and release? 11/15/2018

While both myoglobin and hemoglobin bind oxygen, myoglobin has a higher affinity for oxygen and does not exhibit cooperative binding. This allows myoglobin to release oxygen under conditions where hemoglobin cannot, such as during intense muscle activity.

What is the primary function of the MB gene and its encoded protein? 04/19/2018

The MB gene encodes the protein myoglobin, which is responsible for storing and releasing oxygen in muscle cells, especially in conditions of high oxygen demand.

Customer Reviews (3)

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Reviews
08/05/2020

    Effective in pull-down assay.

    07/01/2020

      Perfect for my experiments.

      07/26/2018

        High-quality product, very satisfied.

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