"LPP" Related Products

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Recombinant Human LPP 293 Cell Lysate

Cat.No.: LPP-4664HCL
Description: Antigen standard for LIM domain containing preferred translocation partner in lipoma (LPP), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: LPP LIM domain containing preferred translocation partner in lipoma [ Homo sapiens ]
Official Symbol: LPP
Synonyms: LPP; LIM domain containing preferred translocation partner in lipoma; lipoma-preferred partner; LIM protein; FLJ30652; FLJ41512; DKFZp779O0231;
Gene ID: 4026
mRNA Refseq: NM_001167671
Protein Refseq: NP_001161143
MIM: 600700
UniProt ID: Q93052
Chromosome Location: 3q27-q28
Pathway: Stabilization and expansion of the E-cadherin adherens junction, organism-specific biosystem;
Function: metal ion binding; protein binding; zinc ion binding;

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