"LCE3D" Related Products

Recombinant Human LCE3D 293 Cell Lysate

Cat.No.: LCE3D-4806HCL
Description: Antigen standard for late cornified envelope 3D (LCE3D) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: LCE3D late cornified envelope 3D [ Homo sapiens ]
Official Symbol: LCE3D
Synonyms: LEP16; SPRL6A; SPRL6B; late cornified envelope protein 3D; late envelope protein 16; small proline rich-like (epidermal differentiation complex) 6A; small proline rich-like (epidermal differentiation complex) 6B; small proline-rich-like epidermal differentiation complex protein 6A; small proline-rich-like epidermal differentiation complex protein 6B
Gene ID: 84648
mRNA Refseq: NM_032563
Protein Refseq: NP_115952
MIM: 612616
UniProt ID: Q9BYE3
Chromosome Location: 1q21

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