"KCNB1" Related Products

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Recombinant Human KCNB1 293 Cell Lysate

Cat.No.: KCNB1-5071HCL
Description: Antigen standard for potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: KCNB1 potassium voltage-gated channel, Shab-related subfamily, member 1 [ Homo sapiens ]
Official Symbol: KCNB1
Synonyms: KCNB1; potassium voltage-gated channel, Shab-related subfamily, member 1; potassium voltage-gated channel subfamily B member 1; Kv2.1; h-DRK1 K(+) channel; potassium channel protein DRK1; delayed rectifier potassium channel 1; delayed rectifier potassium channel Kv2.1; voltage-gated potassium channel subunit Kv2.1; DRK1; KV2.1; h-DRK1;
Gene ID: 3745
mRNA Refseq: NM_004975
Protein Refseq: NP_004966
MIM: 600397
UniProt ID: Q14721
Chromosome Location: 20q13.2
Pathway: Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem; Regulation of Insulin Secretion by Glucagon-like Peptide-1, organism-specific biosystem;
Function: protein binding; voltage-gated ion channel activity; voltage-gated potassium channel activity;

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