Recombinant Human KAT5 293 Cell Lysate

• Cat.No.: KAT5-5088HCL

Specification

• Description: Antigen standard for K(lysine) acetyltransferase 5 (KAT5), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: KAT5 K(lysine) acetyltransferase 5 [ Homo sapiens ]
• Official Symbol: KAT5
• Synonyms: KAT5; K(lysine) acetyltransferase 5; HIV 1 Tat interactive protein, 60kDa , HTATIP; histone acetyltransferase KAT5; cPLA2; ESA1; HTATIP1; K acetyltransferase 5; PLIP; Tat interacting protein; 60kDa; TIP60; K-acetyltransferase 5; cPLA2 interacting protein; lysine acetyltransferase 5; cPLA(2)-interacting protein; 60 kDa Tat-interactive protein; Tat interacting protein, 60kDa; histone acetyltransferase HTATIP; HIV-1 Tat interactive protein, 60kDa; TIP; HTATIP
• Gene ID: 10524
• mRNA Refseq: NM_182709
• Protein Refseq: NP_874368
• MIM: 601409
• UniProt ID: Q92993
• Chromosome Location: 11q13
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; C-MYC pathway, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem
• Function: androgen receptor binding; contributes_to histone acetyltransferase activity; metal ion binding; protein binding; repressing transcription factor binding; transcription coactivator activity; transcription coactivator activity; transferase activity, transferring acyl groups other than amino-acyl groups