"JUP" Related Products

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Recombinant Human JUP 293 Cell Lysate

Cat.No.: JUP-5094HCL
Description: Antigen standard for junction plakoglobin (JUP), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: JUP junction plakoglobin [ Homo sapiens ]
Official Symbol: JUP
Synonyms: JUP; junction plakoglobin; catenin (cadherin associated protein), gamma 80kDa , CTNNG; DP3; DPIII; PDGB; PKGB; catenin gamma; desmoplakin-3; gamma-catenin; desmoplakin III; catenin (cadherin-associated protein), gamma 80kDa; catenin (cadherin-associated protein), gamma (80kD); CTNNG; ARVD12;
Gene ID: 3728
mRNA Refseq: NM_002230
Protein Refseq: NP_002221
MIM: 173325
UniProt ID: P14923
Chromosome Location: 17q21
Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Cell junction organization, organism-specific biosystem;
Function: RPTP-like protein binding; alpha-catenin binding; cadherin binding; protein binding; protein homodimerization activity; protein kinase binding; protein phosphatase binding; structural constituent of cell wall; structural molecule activity; transcription coactivator activity;

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