"ISY1" Related Products

Recombinant Human ISY1 293 Cell Lysate

Cat.No.: ISY1-5142HCL
Description: Antigen standard for ISY1 splicing factor homolog (S. cerevisiae) (ISY1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ISY1 ISY1 splicing factor homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: ISY1
Synonyms: FSAP33; pre-mRNA-splicing factor ISY1 homolog; functional spliceosome-associated protein 33
Gene ID: 57461
mRNA Refseq: NM_020701
Protein Refseq: NP_065752
MIM: 612764
UniProt ID: Q9ULR0
Chromosome Location: 3q21.3
Pathway: Spliceosome, organism-specific biosystem; Spliceosome, 35S U5-snRNP, organism-specific biosystem; Spliceosome, 35S U5-snRNP, conserved biosystem
Function: poly(A) RNA binding; protein binding

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