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Recombinant Human ING2 293 Cell Lysate

Cat.No. : ING2-5208HCL
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  • Gene Information
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Description : Antigen standard for inhibitor of growth family, member 2 (ING2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ING2 inhibitor of growth family, member 2 [ Homo sapiens ]
Official Symbol : ING2
Synonyms : ING2; inhibitor of growth family, member 2; ING1L, inhibitor of growth family, member 1 like; inhibitor of growth protein 2; p33ING2; p32; ING1Lp; inhibitor of growth 1-like protein; ING1L;
Gene ID : 3622
mRNA Refseq : NM_001564
Protein Refseq : NP_001555
MIM : 604215
UniProt ID : Q9H160
Chromosome Location : 4q35.1
Pathway : Senescence and Autophagy, organism-specific biosystem;
Function : DNA binding; chromatin binding; metal ion binding; methylated histone residue binding; phosphatidylinositol binding; protein binding; protein complex binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What were the myocardial expression levels of specific proteins influenced by chronic AngⅡ infusion and Ing2 silencing? 10/08/2022

Chronic AngⅡ infusion increased myocardial expression levels of Ac-p53(Lys382) and p-p53(Ser15), and Ing2 silencing prior to AngⅡ infusion reduced the increase in Ac-p53(Lys382) without affecting p53(Ser15) expression.

What are the expression levels of Ing2 mRNA and protein in mice with chronic Ang Ⅱ infusion? 10/16/2021

Ing2 mRNA and protein levels were significantly higher in mice with chronic Ang Ⅱ infusion compared to saline-infused mice.

What is the overall conclusion drawn from this study? 11/20/2020

The conclusion is that Ing2 silencing can inhibit AngⅡ-induced cardiac remodeling and dysfunction in mice by reducing p53 acetylation.

What was the impact of Ing2 silencing on AngⅡ-induced cardiac function decline? 10/01/2020

Ing2 silencing significantly alleviated AngⅡ-induced cardiac function decline, as evidenced by reduced LVEDD and LVESD and increased LVEF and LVFS.

How does the regulation of p53 acetylation relate to cardiac remodeling and dysfunction? 09/17/2019

The study suggests that the reduction of p53 acetylation by Ing2 silencing contributes to the prevention of cardiac remodeling and dysfunction in response to chronic AngⅡ infusion.

How did chronic infusion of AngⅡ affect left ventricular parameters in mice? 07/05/2019

Chronic AngⅡ infusion led to increased left ventricular end-systolic diameter (LVESD) and left ventricular end-diastolic diameter (LVEDD) and reduced left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) in the mice.

In addition to cardiac function, what other aspects did Ing2 silencing affect in mice with chronic AngⅡ infusion? 04/27/2018

Ing2 silencing improved myocardial mitochondrial damage, reduced myocardial hypertrophy and fibrosis, and inhibited cardiomyocyte apoptosis induced by chronic AngⅡ infusion.

Customer Reviews (3)

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07/30/2022

    Feel free to continue expanding your list by adding these reviews to your Excel sheet.

    07/11/2019

       Consistently achieving specific outcomes validated the pivotal role of the product in our research studies.

      05/08/2016

        The product's unique attributes propelled our research to innovative heights.

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