Recombinant Human ING2 293 Cell Lysate
Cat.No. : | ING2-5208HCL |
- Specification
- Gene Information
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Description : | Antigen standard for inhibitor of growth family, member 2 (ING2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ING2 inhibitor of growth family, member 2 [ Homo sapiens ] |
Official Symbol : | ING2 |
Synonyms : | ING2; inhibitor of growth family, member 2; ING1L, inhibitor of growth family, member 1 like; inhibitor of growth protein 2; p33ING2; p32; ING1Lp; inhibitor of growth 1-like protein; ING1L; |
Gene ID : | 3622 |
mRNA Refseq : | NM_001564 |
Protein Refseq : | NP_001555 |
MIM : | 604215 |
UniProt ID : | Q9H160 |
Chromosome Location : | 4q35.1 |
Pathway : | Senescence and Autophagy, organism-specific biosystem; |
Function : | DNA binding; chromatin binding; metal ion binding; methylated histone residue binding; phosphatidylinositol binding; protein binding; protein complex binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
Ing2-3536M | Recombinant Mouse Ing2 Protein, Myc/DDK-tagged | +Inquiry |
ING2-4543M | Recombinant Mouse ING2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ING2-2090R | Recombinant Rhesus Macaque ING2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ING2-5130H | Recombinant Human ING2 Protein, GST-tagged | +Inquiry |
ING2-580Z | Recombinant Zebrafish ING2 | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionChronic AngⅡ infusion increased myocardial expression levels of Ac-p53(Lys382) and p-p53(Ser15), and Ing2 silencing prior to AngⅡ infusion reduced the increase in Ac-p53(Lys382) without affecting p53(Ser15) expression.
Ing2 mRNA and protein levels were significantly higher in mice with chronic Ang Ⅱ infusion compared to saline-infused mice.
The conclusion is that Ing2 silencing can inhibit AngⅡ-induced cardiac remodeling and dysfunction in mice by reducing p53 acetylation.
Ing2 silencing significantly alleviated AngⅡ-induced cardiac function decline, as evidenced by reduced LVEDD and LVESD and increased LVEF and LVFS.
The study suggests that the reduction of p53 acetylation by Ing2 silencing contributes to the prevention of cardiac remodeling and dysfunction in response to chronic AngⅡ infusion.
Chronic AngⅡ infusion led to increased left ventricular end-systolic diameter (LVESD) and left ventricular end-diastolic diameter (LVEDD) and reduced left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) in the mice.
Ing2 silencing improved myocardial mitochondrial damage, reduced myocardial hypertrophy and fibrosis, and inhibited cardiomyocyte apoptosis induced by chronic AngⅡ infusion.
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Consistently achieving specific outcomes validated the pivotal role of the product in our research studies.
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