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Recombinant Human INF2 293 Cell Lysate

Cat.No. : INF2-5209HCL
  • Specification
  • Gene Information
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Description : Antigen standard for inverted formin, FH2 and WH2 domain containing (INF2), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : INF2 inverted formin, FH2 and WH2 domain containing [ Homo sapiens ]
Official Symbol : INF2
Synonyms : FSGS5; CMTDIE; pp9484; C14orf151; C14orf173; inverted formin-2; HBEBP2-binding protein C; HBEAG-binding protein 2 binding protein C
Gene ID : 64423
mRNA Refseq : NM_032714
Protein Refseq : NP_116103
MIM : 610982
UniProt ID : Q27J81
Chromosome Location : 14q32.33
Function : Rho GTPase binding; actin binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Can INF2 protein’s function affect cytoskeletal organization? 08/01/2022

Yes, INF2 protein’s function modulates cytoskeletal organization, particularly actin filaments.

Does INF2 protein play a role in cell adhesion and migration? 08/27/2021

Yes, INF2 protein’s role might involve regulating cell adhesion and migration processes.

How is INF2 protein’s impact on actin polymerization assessed? 06/21/2021

Research employs techniques like live-cell imaging to study INF2 protein’s effects on actin polymerization.

Can INF2 protein’s role be linked to cellular responses to mechanical forces? 03/27/2020

Yes, INF2 protein’s function may impact cellular responses to mechanical stresses and forces.

Is INF2 protein’s activity regulated by extracellular cues? 09/24/2018

Yes, INF2 protein’s activity can be influenced by extracellular signals and environmental factors.

How is INF2 protein’s involvement studied in cell motility? 08/20/2018

Research aims to understand how INF2 protein’s role contributes to cellular motility and migration.

How does INF2 protein contribute to membrane remodeling? 12/02/2016

INF2 protein participates in membrane remodeling processes, shaping cellular membranes.

Customer Reviews (3)

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Reviews
01/29/2022

    The product's cost-effectiveness and clear instructions were instrumental in our experimental design.

    04/22/2021

      Clear and concise instructions simplified the application of the product, reducing the learning curve.

      02/02/2016

        We appreciated the product’s efficiency in achieving target protein upregulation with minimal time investment.

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