"HSPA12B" Related Products


Recombinant Human HSPA12B 293 Cell Lysate

Cat.No.: HSPA12B-5359HCL
Description: Antigen standard for heat shock 70kD protein 12B (HSPA12B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HSPA12B heat shock 70kD protein 12B [ Homo sapiens ]
Official Symbol: HSPA12B
Synonyms: HSPA12B; heat shock 70kD protein 12B; C20orf60, chromosome 20 open reading frame 60; heat shock 70 kDa protein 12B; dJ1009E24.2; C20orf60; FLJ32150; MGC131912;
Gene ID: 116835
mRNA Refseq: NM_001197327
Protein Refseq: NP_001184256
MIM: 610702
UniProt ID: Q96MM6
Chromosome Location: 20p13
Function: ATP binding; nucleotide binding;

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