"HNF4A" Related Products


Recombinant Human HNF4A 293 Cell Lysate

Cat.No.: HNF4A-5457HCL
Description: Antigen standard for hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HNF4A hepatocyte nuclear factor 4, alpha [ Homo sapiens ]
Official Symbol: HNF4A
Synonyms: HNF4A; hepatocyte nuclear factor 4, alpha; MODY, MODY1, TCF14; hepatocyte nuclear factor 4-alpha; HNF4; NR2A1; TCF-14; HNF-4-alpha; HNF4alpha10/11/12; transcription factor 14; transcription factor-14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1; TCF; MODY; MODY1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha; FLJ39654;
Gene ID: 3172
mRNA Refseq: NM_178849
Protein Refseq: NP_849180
MIM: 600281
UniProt ID: P41235
Chromosome Location: 20q13.12
Pathway: Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; Maturity onset diabetes of the young, organism-specific biosystem; Maturity onset diabetes of the young, conserved biosystem;
Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; activating transcription factor binding; fatty acid binding; fatty-acyl-CoA binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; palmitoyl-CoA hydrolase activity; protein binding; protein homodimerization activity; receptor activity; receptor binding; repressing transcription factor binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; steroid hormone receptor activity; transcription regulatory region DNA binding; zinc ion binding;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.