"HIST1H1E" Related Products

Recombinant Human HIST1H1E 293 Cell Lysate

Cat.No.: HIST1H1E-5551HCL
Description: Antigen standard for histone cluster 1, H1e (HIST1H1E) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HIST1H1E histone cluster 1, H1e [ Homo sapiens ]
Official Symbol: HIST1H1E
Synonyms: HIST1H1E; histone cluster 1, H1e; H1 histone family, member 4 , H1F4, histone 1, H1e; histone H1.4; H1.4; H1e; H1s 4; histone H1b; histone 1, H1e; H1 histone family, member 4; H1E; H1F4; dJ221C16.5; MGC116819;
Gene ID: 3008
mRNA Refseq: NM_005321
Protein Refseq: NP_005312
MIM: 142220
UniProt ID: P10412
Chromosome Location: 6p22.1
Pathway: Activation of DNA fragmentation factor, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Signaling events mediated by HDAC Class III, organism-specific biosystem;
Function: DNA binding; protein binding;

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