"GRAP" Related Products

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Recombinant Human GRAP 293 Cell Lysate

Cat.No.: GRAP-5758HCL
Description: Antigen standard for GRB2-related adaptor protein (GRAP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GRAP GRB2-related adaptor protein [ Homo sapiens ]
Official Symbol: GRAP
Synonyms: GRAP; GRB2-related adaptor protein; GRB2-related adapter protein; growth factor receptor-bound protein 2-related adaptor protein; MGC64880;
Gene ID: 10750
mRNA Refseq: NM_006613
Protein Refseq: NP_006604
MIM: 604330
UniProt ID: Q13588
Chromosome Location: 17p11.2
Pathway: Kit Receptor Signaling Pathway, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by SCF-KIT, organism-specific biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem;
Function: SH3/SH2 adaptor activity;

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