Recombinant Human GPR18 293 Cell Lysate GPR18-5792HCL

Recombinant Human GPR18 293 Cell Lysate

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Recombinant Human GPR18 293 Cell Lysate

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Cat.No. : GPR18-5792HCL
Description : Antigen standard for G protein-coupled receptor 18 (GPR18), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : GPR18 G protein-coupled receptor 18 [ Homo sapiens ]
Official Symbol : GPR18
Synonyms : GPR18; G protein-coupled receptor 18; N-arachidonyl glycine receptor; NAGly receptor; G-protein coupled receptor 18;
Gene ID : 2841
mRNA Refseq : NM_001098200
Protein Refseq : NP_001091670
MIM : 602042
UniProt ID : Q14330
Chromosome Location : 13q32
Pathway : Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; GPCRs, Other, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function : G-protein coupled purinergic nucleotide receptor activity; G-protein coupled receptor activity; receptor activity; signal transducer activity;

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