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Recombinant Human GLS2 293 Cell Lysate

Cat.No.: GLS2-5895HCL
Description: Antigen standard for glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GLS2 glutaminase 2 (liver, mitochondrial) [ Homo sapiens ]
Official Symbol: GLS2
Synonyms: GLS2; glutaminase 2 (liver, mitochondrial); glutaminase liver isoform, mitochondrial; GA; GLS; hLGA; LGA; L-glutaminase; glutaminase I; glutaminase GA; breast cell glutaminase; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase; MGC71567;
Gene ID: 27165
mRNA Refseq: NM_013267
Protein Refseq: NP_037399
MIM: 606365
UniProt ID: Q9UI32
Chromosome Location: 12q13
Pathway: Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; D-Glutamine and D-glutamate metabolism, organism-specific biosystem; D-Glutamine and D-glutamate metabolism, conserved biosystem;
Function: glutaminase activity; hydrolase activity; protein binding;

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