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Recombinant Human GIPC1 293 Cell Lysate

Cat.No.: GIPC1-5928HCL
Description: Antigen standard for GIPC PDZ domain containing family, member 1 (GIPC1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GIPC1 GIPC PDZ domain containing family, member 1 [ Homo sapiens ]
Official Symbol: GIPC1
Synonyms: GIPC1; GIPC PDZ domain containing family, member 1; C19orf3, chromosome 19 open reading frame 3 , regulator of G protein signalling 19 interacting protein 1 , RGS19IP1; PDZ domain-containing protein GIPC1; GIPC; GLUT1CBP; Hs.6454; NIP; SEMCAP; SYNECTIN; TIP 2; tax interaction protein 2; RGS19-interacting protein 1; RGS-GAIP-interacting protein; GLUT1 C-terminal binding protein; GAIP C-terminus-interacting protein; IGF-1 receptor interacting protein 1; regulator of G-protein signalling 19 interacting protein 1; IIP-1; TIP-2; C19orf3; RGS19IP1; SYNECTIIN; MGC3774; MGC15889;
Gene ID: 10755
mRNA Refseq: NM_005716
Protein Refseq: NP_005707
MIM: 605072
UniProt ID: O14908
Chromosome Location: 19p13.1
Pathway: Neurotrophic factor-mediated Trk receptor signaling, organism-specific biosystem; Syndecan-4-mediated signaling events, organism-specific biosystem; TGF-beta Receptor Signaling Pathway, organism-specific biosystem;
Function: PDZ domain binding; actin binding; myosin binding; protein binding; protein homodimerization activity; receptor binding;

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