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Recombinant Human GHRL 293 Cell Lysate

Cat.No.: GHRL-5941HCL
Description: Antigen standard for ghrelin/obestatin prepropeptide (GHRL), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GHRL ghrelin/obestatin prepropeptide [ Homo sapiens ]
Official Symbol: GHRL
Synonyms: GHRL; ghrelin/obestatin prepropeptide; ghrelin, growth hormone secretagogue receptor ligand; appetite-regulating hormone; ghrelin; MTLRP; obestatin; motilin-related peptide; growth hormone secretagogue; ghrelin/obestatin preprohormone; growth hormone-releasing peptide;
Gene ID: 51738
mRNA Refseq: NM_001134941
Protein Refseq: NP_001128413
MIM: 605353
UniProt ID: Q9UBU3
Chromosome Location: 3p26-p25
Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem;
Function: G-protein coupled receptor binding; ghrelin receptor binding; ghrelin receptor binding; growth hormone-releasing hormone activity; growth hormone-releasing hormone activity; growth hormone-releasing hormone activity; protein tyrosine kinase activator activity;

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