"FRK" Related Products

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Recombinant Human FRK 293 Cell Lysate

Cat.No.: FRK-6137HCL
Description: Antigen standard for fyn-related kinase (FRK) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FRK fyn-related kinase [ Homo sapiens ]
Official Symbol: FRK
Synonyms: FRK; fyn-related kinase; PTK5, PTK5 protein tyrosine kinase 5; tyrosine-protein kinase FRK; GTK; RAK; protein-tyrosine kinase 5; PTK5 protein tyrosine kinase 5; nuclear tyrosine protein kinase RAK; PTK5;
Gene ID: 2444
mRNA Refseq: NM_002031
Protein Refseq: NP_002022
MIM: 606573
UniProt ID: P42685
Chromosome Location: 6q21-q22.3
Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding;

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