"FERD3L" Related Products

Recombinant Human FERD3L 293 Cell Lysate

Cat.No.: FERD3L-6263HCL
Description: Antigen standard for Fer3-like (Drosophila) (FERD3L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FERD3L?Fer3-like bHLH transcription factor [?Homo sapiens?(human) ]
Official Symbol: FERD3L
Synonyms: FERD3L; NATO3; NTWIST; N-TWIST; bHLHa31; Fer3-like bHLH transcription factor; fer3-like protein; neuronal twist; nephew of atonal 3; basic helix-loop-helix protein N-twist; class A basic helix-loop-helix protein 31
Gene ID: 222894
mRNA Refseq: NM_152898
Protein Refseq: NP_690862
UniProt ID: Q96RJ6
Chromosome Location: 7p21.1
Function: DNA binding; protein dimerization activity

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