Recombinant Human FAM78A 293 Cell Lysate

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Recombinant Human FAM78A 293 Cell Lysate

FAM78A Related Products

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Cat.No. : FAM78A-6349HCL
Description : Antigen standard for family with sequence similarity 78, member A (FAM78A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : FAM78A family with sequence similarity 78, member A [ Homo sapiens ]
Official Symbol : FAM78A
Synonyms : FAM78A; family with sequence similarity 78, member A; C9orf59, chromosome 9 open reading frame 59; protein FAM78A; FLJ00024; C9orf59; RP11-544A12.6;
Gene ID : 286336
mRNA Refseq : NM_033387
Protein Refseq : NP_203745
UniProt ID : Q5JUQ0
Chromosome Location : 9q34

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