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Recombinant Human ETS2 293 Cell Lysate

Cat.No.: ETS2-6525HCL
Description: Antigen standard for v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) (ETS2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ETS2 v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) [ Homo sapiens ]
Official Symbol: ETS2
Synonyms: ETS2; v-ets erythroblastosis virus E26 oncogene homolog 2 (avian); v ets avian erythroblastosis virus E26 oncogene homolog 2; protein C-ets-2; oncogene ETS-2; v-ets avian erythroblastosis virus E2 oncogene homolog 2; v-ets avian erythroblastosis virus E26 oncogene homolog 2; ETS2IT1;
Gene ID: 2114
mRNA Refseq: NM_001256295
Protein Refseq: NP_001243224
MIM: 164740
UniProt ID: P15036
Chromosome Location: 21q22.3
Pathway: C-MYB transcription factor network, organism-specific biosystem; Dorso-ventral axis formation, organism-specific biosystem; Dorso-ventral axis formation, conserved biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL-2 Signaling Pathway, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem;
Function: DNA binding; glucocorticoid receptor binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity;

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