"ERP44" Related Products

Recombinant Human ERP44 293 Cell Lysate

Cat.No.: ERP44-6543HCL
Description: Antigen standard for endoplasmic reticulum protein 44 (ERP44) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ERP44 endoplasmic reticulum protein 44 [ Homo sapiens ]
Official Symbol: ERP44
Synonyms: ERP44; endoplasmic reticulum protein 44; thioredoxin domain containing 4 (endoplasmic reticulum) , TXNDC4; endoplasmic reticulum resident protein 44; KIAA0573; PDIA10; protein disulfide isomerase family A; member 10; ER protein 44; thioredoxin domain-containing protein 4; endoplasmic reticulum resident protein 44 kDa; protein disulfide isomerase family A, member 10; thioredoxin domain containing 4 (endoplasmic reticulum); TXNDC4;
Gene ID: 23071
mRNA Refseq: NM_015051
Protein Refseq: NP_055866
MIM: 609170
UniProt ID: Q9BS26
Chromosome Location: 9q22.33
Function: protein disulfide isomerase activity;

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